Harvey et al., “ A genetically encoded fluorescent sensor of ERK activity,” Proc. Covert, “ High-sensitivity measurements of multiple kinase activities in live single cells,” Cell 157, 1724– 1734 (2015). Griffith, “ Sustained epidermal growth factor receptor levels and activation by tethered ligand binding enhances osteogenic differentiation of multi-potent marrow stromal cells,” J. Masters, “ Immobilized epidermal growth factor stimulates persistent, directed keratinocyte migration via activation of PLCγ1,” FASEB J. Lauffenburger, “ RAS mutations impact TNF-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects,” Cancer Res. Shi et al., “ Conservation of protein abundance patterns reveals the regulatory architecture of the EGFR-MAPK pathway,” Sci. Wolf-Yadlin et al., “ Effects of HER 2 overexpression on cell signaling networks governing proliferation and migration,” Mol. Muta et al., “ Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine,” Nat. Rodland et al., “ Multiple mechanisms are responsible for transactivation of the epidermal growth factor receptor in mammary epithelial cells,” J. Keyes et al., “ Signaling diversity enabled by Rap1-regulated plasma membrane ERK with distinct temporal dynamics,” eLife 9, e57410 (2020). Wan, “ EGF-induced cell migration is mediated by ERK and PI 3K/AKT pathways in cultured human lens epithelial cells,” J. Lauffenburger, “ Parsing ERK activation reveals quantitatively equivalent HER 2 in human mammary epithelial cells,” J. Our results demonstrate that context impacts ERK dynamics and that comparison of distinct contexts can be used to elucidate new aspects of the cell signaling network.
Through experimental analysis we identified growth differentiation factor-15 as a candidate factor that led to extended ERK activation through a protein kinase C-α/β dependent pathway. The activity in microfluidic culture resembled that of the control condition, suggesting that shear stress led to the early activity and a loss of autocrine factors dampened extended signaling. Using a single-cell ERK kinase translocation reporter, we observed extended ERK activation in well culture and only transient activity in microfluidic culture. Here, we compared extracellular-regulated kinase (ERK) signaling in response to epidermal growth factor treatment of human mammary epithelial cells cultures in either well culture or a microfluidic device. These signaling patterns are also impacted by the larger context that the cell experiences (i.e., stimuli such as media formulation or substrate stiffness that are constant in an experiment exploring a particular variable but may differ between independent experiments which explore that variable) although the reason for different dynamics is not always obvious. : imagescience/utility/ImageScienceĪt .run(MyEdges.java:47)Īt .run(MyGradient.java:74)Īt .applyEnhanceChromocenters(ChromocentersEnhancement.java:32)Īt _.run(ChromocenterSegmentationBatchPlugin_.java:65)Īt ij.nCommand(Executer.java:137)Ĭreation of image of the CC without no image, that is due at nucleusJ which is working with the version of MorpholibJ_-1.2.2 or anterior.Cellular signaling dynamics are sensitive to differences in ligand identity, levels, and temporal patterns. New version of nucleusJ was realised to correct this error:
#Imagej citation plus#
To use nucleusJ you need to dowload the two depencies available here plus jama.jar. Index bounds error similar to the previous in the version 1.0.2 IEEE Transactions on Pattern Analysis and Machine Intelligence 13, 583–598. Watersheds in digital spaces: an efficient algorithm based on immersion simulations. A threshold selection method from gray-level histograms. Proceedings of the National Academy of Sciences 99, 14584 –14589. Interphase chromosomes in Arabidopsis are organized as well defined chromocenters from which euchromatin loops emanate. Mathematical Morphology in Image Processing.įransz, P., de Jong, J.H., Lysak, M., Castiglione, M.R., Schubert, I., 2002. The morphological approach to segmentation: the watershed transformation. International workshop on image processing, real-time edge and motion detection.īeucher, S., Meyer, F., 1993. Statistical Analysis of 3D Images Detects Regular Spatial Distributions of Centromeres and Chromocenters in Animal and Plant Nuclei. Andrey, P., Kiêu, K., Kress, C., Lehmann, G., Tirichine, L., Liu, Z., Biot, E., Adenot, P.-G., Hue-Beauvais, C., Houba-Hérin, N., Duranthon, V., Devinoy, E., Beaujean, N., Gaudin, V., Maurin, Y.,Debey, P., 2010.
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